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KMID : 0613820020120060682
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Journal of Life Science
2002 Volume.12 No. 6 p.682 ~ p.687
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Characterization of the enzymatic property of thermostable carboxypeptidase Taq by addition of metal ions and replacement of active center metal
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Lee Sang-Hyun
Ha Jong-Myuong
Ha Bae-Jin
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Abstract
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We analyzed improvement on the enzyme activity of CPase Taq by addition of various metal ions. The enzyme activity was increased more then four times by 1 mM cobalt ion and almost three times by 1 mM calcium ion. However, the active center metal zinc ion did not affect the enzyme activity. In order to investigate whether the active center metal affects the enzyme activity, zinc ion which is occupied the active center of the enzyme was replaced by cobalt ion which activates the enzyme activity very effectively. Since the cobalt ion in the active center of the cobalt-substituted CPase Taq did not affect the enzyme activity, it could act as the native metal ion in the active center of the enzyme.
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KEYWORD
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carboxypeptidase, zinc-dependent, thermostable, cobalt-substituted
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